Ron Lab
Lab protocols
Heather Harding's protocol for detection of PERK or GCN2 protein in cell lysates or tissue extracts by combined immunoprecipitation western immunobloting (download pdf)
Heather Harding's protocol for detection of phosphorylated eIF2α by western blotting (download pdf)
Heather Harding & Huiqing Zeng's protocol for isolating nuclear and cytoplasmic extracts
Heather Harding's protocol for ES cell culture
Choosing template DNA to construct a targeting vector for homologous recombination in ES cells
Culturing cells with defective ISR
Anne Bertolotti's protocol for culture of AR42J cells (download pdf)
Heather Harding's protocol for immortalizing MEFs with SV-40 large T antigen (download pdf)
Marcie Calfon's & Heather Harding's protocol for detection of in vivo DCF fluorescence in C. elegans
Cristina Benedetti's protocol for genotyping dead eggs of C. elegans
How to find 129sv BACs for your gene
Heather Hardings protocol for producing VSV-G pseudotyped retroviruses
How to obtain full-length cDNA clones
Protocols related to mutant mice
Obtaining mice from our collection
Detection of AIRAP genotype in knockout mice
Detection of Alb::A1 (A.K.A. Alb::GADD34(+) transgene)
Detection of ATF4 genotype (Leiden's mutation) and wildtype
Detection of CHOP genotype in knockout mice
Detection of CRE in transgenic mice
On genotyping ERO1β mutant mice
Detection of GADD34 genotype in knockout mice (WT & KO1 Ex allele)
Detection of GCN2 genotype in KO4 knockout mice and cells
Detection of IRE1α genotype in knockout mice and cell lines
Detection of IRE1β genotype in knockout mice
Detection of P58ipk/DnajC3 genotype in knockout mice
Detection of PERK genotype in knockout mice and cell lines