Ron Lab



Lab protocols

Heather Harding's protocol for detection of PERK or GCN2 protein in cell lysates or tissue extracts by combined immunoprecipitation western immunobloting (download pdf)

Heather Harding's protocol for detection of phosphorylated eIF2α by western blotting (download pdf)

Heather Harding & Huiqing Zeng's protocol for isolating nuclear and cytoplasmic extracts

Heather Harding's protocol for ES cell culture

Choosing template DNA to construct a targeting vector for homologous recombination in ES cells

Culturing cells with defective ISR

Anne Bertolotti's protocol for culture of AR42J cells (download pdf)

Heather Harding's protocol for immortalizing MEFs with SV-40 large T antigen (download pdf)

Marcie Calfon's & Heather Harding's protocol for detection of in vivo DCF fluorescence in C. elegans

Marcie Calfon's & Heather Harding's current protocols for detection of XBP-1 processing in mouse or human cells

Heather Harding's protocol for cleaning worms with bleach, includes Arza Ron's procedure for neutralizing the bleach with thiosulfate

Cristina Benedetti's protocol for genotyping dead eggs of C. elegans

How to find 129sv BACs for your gene

Heather Hardings protocol for producing VSV-G pseudotyped retroviruses

How to obtain full-length cDNA clones

Protocols related to mutant mice

Obtaining mice from our collection

Detection of AIRAP genotype in knockout mice

Detection of Alb::A1 (A.K.A. Alb::GADD34(+) transgene)

Detection of ATF4 genotype (Leiden's mutation) and wildtype

Detection of CHOP genotype in knockout mice

Detection of CRE in transgenic mice

On genotyping ERO1β mutant mice

Detection of GADD34 genotype in knockout mice (WT & KO1 Ex allele)

Detection of GCN2 genotype in KO4 knockout mice and cells

Detection of IRE1α genotype in knockout mice and cell lines

Detection of IRE1β genotype in knockout mice

Detection of P58ipk/DnajC3 genotype in knockout mice

Detection of PERK genotype in knockout mice and cell lines